| Application | RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy's 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. |
| Other Notes | Modified for autoclaving
Without L-glutamine and sodium bicarbonate. |
| Legal Information | Auto-Mod is a trademark of the Sigma-Aldrich Corporation. |
| Quantity | Formulated to contain 10.3 grams of powder per liter of medium. |
| Reconstitution | Supplement with 0.3 L-glutamine, 2.0 g/L sodium bicarbonate. |
| | Auto-Mod is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
